学习与工作经历：

2007.09-2012.06，郑州大学，临床医学系，本科

2012.07-2015.06，中南大学，湘雅医院，硕士

2015.07-2019.06，中南大学，湘雅医院，博士

2016.11-2018.10，美国约翰霍普金斯大学，联合培养博士

2019.07-2025.04，郑州大学第一附属医院，骨科，医师/主治医师

2025.05-至今，山东大学齐鲁第二医院，副主任医师

研究方向：

脊柱脊髓疾病的临床与基础研究

学术/社会兼职：

兼任美国骨科学会（ORS）会员

国际华人骨研究学会（ICMRS）会员

美国骨矿化研究学会（ASBMR）会员

山东省医师协会快速康复分会常委

中国医师协会神经修复专委会基础与转化学组委员

全国卫生产业企业管理协会脊柱健康分会委员

山东省医学会骨科分会学术发展学组委员

获奖荣誉：

国际华人骨研究学会（ICMRS) Webste Jee Young Investigator Award奖

美国骨矿化研究学会（ASBMR）Young Investigator Award奖

本科生课程：

研究生课程：

1、82472508，感觉神经来源SP通过mrgprB2/PLCβ通路激活肥大细胞进而影响破骨细胞活性导致终板退变的机制研究，国家自然科学基金面上项目，2025.01-2028.12，49万元，主持

2、82002353，巨噬细胞衰老通过IL-10/JAK1/STAT3通路介导免疫-血管沟通导致软骨终板硬化的机制研究，国家自然科学基金青年基金项目，2021.01-2023.12，24万元，主持

3、tsqn202507349，山东省泰山学者青年专家，37.5万元，主持

1.Yonggang Fan,Weixin Zhang,Xiusheng Huang,Mingzhe Fan,Chenhao Shi,Lantian Zhao,Guofu Pi,Huafeng Zhang,Shuangfei Ni*.Senescent-like macrophages mediate angiogenesis for endplate sclerosis via IL-10 secretion in male mice.Nat Commun. 2024 Apr 5;15(1):2939. doi: 10.1038/s41467-024-47317-1.

2.Shuangfei Ni,Zemin Ling,Xiao Wang,Yong Cao,Tianding Wu,Ruoxian Deng,Janet L Crane,Richard Skolasky,Shadpour Demehri,Gehua Zhen,Amit Jain,Panfeng Wu,Dayu Pan,Bo Hu,Xiao Lyu,Yusheng Li,Hao Chen,Huabin Qi,Yun Guan,Xinzhong Dong,Mei Wan,Xuenong Zou,Hongbin Lu,Jianzhong Hu*,Xu Cao*. Sensory Innervation in Porous Endplates by netrin-1 from Osteoclasts mediates PGE2-induced Spinal Hypersensitivity in Mice.Nat Commun.2019 Dec 10;10(1):5643. doi: 10.1038/s41467-019-13476-9.

3.Shuangfei Ni#,Zixiang Luo#,Liyuan Jiang,Zhu Guo,Ping Li,Xiang Xu,Yong Cao,Chunyue Duan,Tianding Wu,Chengjun Li,Hongbin Lu*,Jianzhong Hu*. UTX/KDM6A Deletion Promotes Recovery of Spinal Cord Injury by Epigenetically Regulating Vascular Regeneration.Mol Ther.2019 Dec 4;27(12):2134-2146. doi: 10.1016/j.ymthe.2019.08.009.

4.Liwei Zheng#,Yong Cao#,Shuangfei Ni#,Huabin Qi,Zemin Ling,Xin Xu,Xuenong Zou,Tianding Wu,Ruoxian Deng,Bo Hu,Bo Gao,Hao Chen,Yusheng Li,Jianxi Zhu,Francis Tintani,Shadpour Demehri,Amit Jain,Khaled M Kebaish,Shenghui Liao,Cheryle A Séguin,Janet L Crane,Mei Wan,Hongbin Lu,Paul D Sponseller,Lee H Riley 3rd,Xuedong Zhou,Jianzhong Hu,Xu Cao*.Ciliary parathyroid hormone signaling activates transforming growth factor-βto maintain intervertebral disc homeostasis during aging. Bone Res. 2018 Jul 18;6:21.

5.Shuangfei Ni#,Xiusheng Huang#,Xuesen Li#,Chenhao Shi,Mingzhe Fan,Lantian Zhao,Zijie Rong*,Huafeng Zhang*. METTL3 promotes nucleus pulposus cell senescence in intervertebral disc degeneration by regulating TLR2 m6A methylation and gut microbiota. J Gerontol A Biol Sci Med Sci. 2024 Jun 10:glae150. doi: 10.1093/gerona/glae150.

6.Zi-Jie Rong#,Min Chen#,Hong-Hua Cai,Gui-Hua Liu,Jin-Biao Chen,Hao Wang,Zhi-Wen Zhang,Yu-Liang Huang*,Shuang-Fei Ni*. Ursolic acid molecules dock MAPK1 to modulate gut microbiota diversity to reduce neuropathic pain. Neuropharmacology. 2024 Jul 1;252:109939. doi: 10.1016/j.neuropharm.2024.109939.

7.Chenhao Shi,Yonggang Fan,Xiusheng Huang,Mingzhe Fan,Lantian Zhao,Huafeng Zhang*,Shuangfei Ni*. Single-cell RNA sequencing reveals the differentiation and regulation of endplate cells in human intervertebral disc degeneration. Sci Rep. 2024 Sep 13;14(1):21414. doi: 10.1038/s41598-024-71891-5.

8.Shuangfei Ni#,Zixiang Luo#,Yonggang Fan,Weixin Zhang,Wei Peng*,Huafeng Zhang*. Alteration of m6A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice. Front Neurosci. 2022 Aug 25;16:904573. doi: 10.3389/fnins.2022.904573. eCollection 2022.

9.Shuangfei Ni, Bo Yang, Lei Xia, Huafeng Zhang*. EZH2 mediates miR-146a-5p/HIF-1αto alleviate inflammation and glycolysis after acute spinal cord injury, Mediators of Inflammation.2021 May 19;2021:5591582.doi: 10.1155/2021/5591582.

10.Yonggang Fan,Shuangfei Ni*, Huafeng Zhang*. Associations of Copper Intake with Bone Mineral Density and Osteoporosis in Adults: Data from the National Health and Nutrition Examination Survey. Biological Trace Element Research.2022 May;200(5):2062-2068. doi:10.1007/s12011-021-02845-5.

11.Yonggang Fan,Shuangfei Ni*, Huafeng Zhang*.Association between Healthy Eating Index-2015 total and component food scores with osteoporosis in middle-aged and older Americans: a cross-sectional study with U.S. National Health and Nutrition Examination Survey.Osteoporos Int. 2022 Apr;33(4):921-929. doi: 10.1007/s00198-021-06247-0.

12.Shuangfei Ni,Yong Cao,Shenghui Liao,Chunyue Duan,Liyuan Jiang,Jianzhong Hu,Hongbin Lu,Tianding Wu*. Unilateral Osteotomy of Lumbar Facet Joint Induces a Mouse Model of Lumbar Facet Joint Osteoarthritis.Spine (Phila Pa 1976).2019 Aug 15;44(16):E930-E938.

13.Shuangfei Ni,Yong Cao,Liyuan Jiang,Zixiang Luo,Hongbin Lu,Jianzhong Hu,Tianding Wu*.Synchrotron Radiation Imaging Reveals the Role of Estrogen in Promoting Angiogenesis After Acute Spinal Cord Injury in Rats.Spine (Phila Pa 1976). 2018 Sep 15;43(18):1241-1249.

14.Tianding Wu#,Shuangfei Ni#,Yong Cao#,Shenghui Liao,Jianzhong Hu,Chunyue Duan*.Three-dimensional visualization and pathologic characteristics of cartilage and subchondral bone changes in the lumbar facet joint of an ovariectomized mouse model.Spine J. 2018 Apr;18(4):663-673.

15.Shenghui Liao#,Shuangfei Ni#,Yong Cao#,Xianzhen Yin,Tianding Wu*,Hongbin Lu,Jianzhong Hu,Hao Wu,Ye Lang.The 3D characteristics of post-traumatic syringomyelia in a rat model: a propagation-based synchrotron radiation microtomography study.J Synchrotron Radiat. 2017 Nov 1;24(Pt 6):1218-1225.